Abstract
A simple direct micro flame photometric determination for tissue calcium was developed. Approximately 100 mg fresh tissue containing a total of 0.5–3.0 μg Ca was extracted with trichloroacetic acid solution, and the extract was analyzed after the addition of 5 meq Na/l, 1 meq K/l, and isopropyl alcohol. Under these conditions tissue sodium, potassium, and phosphate did not cause significant interference. The values and standard errors for fresh rat liver, heart, kidney, and diaphragm muscle were 3.96 ± 0.11, 3.95 ± 0.06, 5.52 ± 0.18, and 4.83 ± 0.23 μg Ca 100 mg , respectively. Good agreement with the results of analysis by the Clark and Collip method was obtained.
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