The direct effect of 1,25-dihydroxycholecalciferol on membrane phospholipid composition and phosphate transport.

Abstract

Current evidence suggests that the mechanism of action of several hormones may be mediated through alterations in membrane lipid composition1–4. Both the content and the degree of fatty acid unsaturation of phosphatidylcholine (PC) in renal proximal tubular and duodenal brush border membranes is increased after 1,25-dihydroxycholecalciferol (1,25(OH)2D3) administration to animals5,6. In both the intestine5’7 and the kidney8 indirect evidence suggests that these lipid changes may be important in stimulating calcium and phosphate transport. In recent studies performed in this laboratory fluorescent phospholipid derivatives have been used to demonstrate that 1,25(OH)2D3 rapidly and directly stimulates the transfer of PC from liposomes to renal brush border membrane vesicles (BBMV)9. The following studies were performed to investigate the effects of 1,25(OH)2D3 induced membrane phospholipid alterations on phosphate transport.KeywordsBrush Border MembranePhosphate TransportPhosphate UptakeBrush Border Membrane VesicleRenal Brush Border MembraneThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.