Abstract
Thyroid and steroid hormones bind to chromatin-associated nuclear protein receptors which appear to be hormone specific and are believed to mediate the action of these hormones through synthesis of mRNA. The purpose of this investigation was to test the hypothesis of hormonal control through regulation of specific gene expression. Rat GH and PRL were chosen as a model, since previous work has indicated that the synthesis of these hormones is regulated by thyroid hormone and estrogen, respectively. Male rats were kept intact (N) or were thyroidectomized (Tx) and treated with estradiol (N + E or Tx + E) or replaced with T4, (Tx + T4). GH and PRL synthesis was determined in quarter pituitaries by the in vitro incorporation of [3H]leucine into specific immunoprecipitates. GH and PRL mRNA activity was measured by cell-free translation of total RNA extracted from the remaining pituitary tissue. Tx suppressed both GH synthesis and mRNA activity to 2.8% and 1.4% of the level in N rats and was not affected by trea...
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