Abstract
ABSTRACTTwo polyclonal antisera to plum pox virus isolates, PPV-C and PPV-A, raised after a single immunization were collected at different stages after injection and serologically studied using electro-blot-immunoassay and indirect ELISA. Antisera, collected one week after the single injection, reacted strongly with the respective homologous capsid protein, but weakly or not at all with the core capsid protein. Batches of both antisera obtained 3, 4, 5, 6 and 7 weeks after injection reacted as well as with the core capsid proteins, indicating a continuously increase in the proportion of antibodies to the core capsid proteins. Comparing the antisera collected 1 week after immunization and N-termini antibodies to PPV-C and PPV-A, respectively, demonstrated that both types of antisera possess strain specificity and confirmed our previously findings on the serological relationships between the PPV isolates studied.
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