Abstract
Objective To explore the differential expression profiling of microRNAs(miRNAs)in cutaneous wounds between diabetic rats and normal rats, and further investigate its significance in wound healing. Methods The diabetic rat models were induced by intraperitoneal injection of 65 mg/kg of streptozotocin(STZ); after four weeks, create full-thickness excisional dorsal wounds in 6 established diabetic model rats and normal rats respectively; then wound tissues were harvested on day 3.Use Trizol to extract total RNA from the sample; purify it after quality inspection qualified; then to the procedures of sample labeling and hybridization. Now hybrid images can be obtained through scanning the washed microarray slides by Agilent Scanner. Analyze these images and extract the data by agilent feature extraction software; then normalize and analyze the data by agilent genespring software. The miRNA profiling results were validated by real- time reverse transcriptase polymerase chain reaction(Real-time PCR). Results A total of 83 miRNAs were differentially expressed in cutaneous wounds of diabetic rats compared with normal rats, among which 18 were up-regulated while 65 are down-regulated. The significantly up-regulated miRNAs were rno-miR-496-5p, rno-miR-105, rno-miR-122-5p,etc;and the significantly down-regulated miRNAs were rno-miR-196a-5p, rno-miR-134-5p, rno-miR-31-3p, etc.The results of Real-time PCR have good consistency with the microarray results.KEGG pathways enrichment analysis shows that miR-31, miR- 222, miR-449a and other significant differential expressed miRNAs were respectively involved in mitogen-activated protein kinase(MAPK), Wnt, Notch and other signaling pathways which link to wound healing. Conclusion There was significant differential miRNAs expression in cutaneous wounds between diabetic rats and normal rats, which may be closely related to the mechanisms of diabetic wound healing. Key words: Diabetes mellitus; Wound healing; MicroRNAs; Differential expression
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
