The different effects of intramuscularly-injected lactate on white and brown adipose tissue in vivo.

Abstract

Lactate is an important product of glycolysis metabolism during exercise and has long been recognized as an important metabolic signaling molecule involved in inhibiting lipolysis and promoting lipogenesis, which consequently leads to regulated adipose tissue metabolism. However, recent studies have shown that lactate promotes the browning of white adipose tissue (WAT), which induces heat production and energy expenditure and ultimately causes weight loss. These studies assessing the effects of lactate on lipid metabolism in adipose tissue have revealed conflicting data, making it an important area worthy of further research. In this study, using intramuscular injection of lactate to the gastrocnemius, weidentified the role of lactate treatment on lipid metabolism and mitochondrial biogenesis of white adipose tissue and brown adipose tissue (BAT). Our results showed that lactate treatment activated the cAMP/PKA signaling pathway and promoted the expression of lipolysis-related proteins (AMPK, HSL, ATGL) and mitochondrial biomarkers (PGC-1α, COXIV) of WAT, while BAT showed an opposite trend after lactate treatment. Further studies showed that lactate treatment significantly increased serum epinephrine and promoted β3-AR protein expression in WAT and significantly decreased in BAT. Our study shows that lactate seems to regulate β3-adrenergic receptors differently in WAT and BAT, thereby eliciting disparate responses in adipose tissue.