The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart

Xifeng Yang,Shihao Chen,Shengli Yin,Yunqi Liu,Jiaxin Chen,Daping Quan,Ying Bai

doi:10.3390/app11177768

Xifeng Yang, Shihao Chen + Show 5 more

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https://doi.org/10.3390/app11177768

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Abstract

Injectable decellularized matrix hydrogels derived from either myocardium or small intestinal submucosa (pDMYO-gel, pDSIS-gel) have been successfully used for myocardial injury repair. However, the relationship between tissue-specific biological functions and protein composition in these two materials is not clear yet. In this study, the protein composition, mechanical properties, and morphology of these two hydrogels and their effects on the behavior of neonatal rat cardiomyocytes (NRCMs) and human umbilical vein endothelial cells (HUVECs), are investigated. The results show that pDMYO-gel is more conducive to growth, adhesion, spreading, and maintenance of normal NRCM beating, due to its higher proportion of extracellular matrix (ECM) glycoproteins (49.55%) and some unique functional proteins such as annexin-6 (ANXA6), agrin (AGRN), cathepsin D (CTSD) and galectin-1 (LGALS1), whereas pDSIS-gel is more conducive to the proliferation of HUVECs. Animal study shows that pDMYO-gel has a better effect on improving cardiac function, inhibiting myocardial fibrosis and maintaining ventricular wall thickness in acute myocardial infarction models in vivo. Therefore, it is proposed that injectable pDMYO-gel hydrogel may be more suitable for functional recovery of myocardial injuries.

Highlights

Myocardial infarction is one of the most common coronary heart diseases
The decellularization of porcine myocardial and small intestinal submucosa tissues were performed according to previously reported methods [11,19] (Figure 1A)
The above results indicate that porcine decellularized myocardial matrix (pDMYO)-gel has a better effect on improving cardiac function, inhibiting myocardial fibrosis, and maintaining ventricular wall thickness after acute myocardial infarction in rats, which is consistent with the results of cell culture study in vitro that pDMYO-gel is more conducive to maintaining myocardial cell viability

Summary

Introduction

Inflammatory cells infiltrate the infarct area, and secrete matrix metalloproteinases and other active degradation substances that degrade the extracellular matrix, leading to replacement of the infarct area by scar fibrous tissues. Biomaterials provide new options for the treatment of myocardial injuries after myocardial infarction. These materials mainly include synthetic [1,2,3], natural [4,5,6,7,8], and biological materials [9,10,11]. Biological tissue-derived decellularized matrices have excellent cell affinity due to their large content in bioactive substances, such as collagen, glycosaminoglycans (GAG), proteoglycans, various growth factors, and other bioactive components, all promoting specific cell growth, differentiation, endogenous tissue regeneration, and repair. Porcine decellularized small intestinal submucosa matrix (pDSIS), porcine decellularized myocardial matrix (pDMYO), and human decellularized amniotic membrane/human decellularized placenta (HDAM/HDP)

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