Abstract
Hepatitis C virus (HCV) has infected about 170-200 million people worldwide and HCV genotypes have been related with a particular geographic distribution. The information of genotypes in chronic hepatitis C is critical for the decision of the pharmaceutical regimen, and for the therapeutic outcome. The aim of the study was determining the dispersion of hepatitis C Virus genotype in our area. The study was conducted on 42 patients who referred from various clinics infected with HCV. Serum HCV RNA levels were quantified by using the COBAS AMPLICOR HCV Monitor 2.0. An 851 bp long fragment spanning codons 63 to 347 of the RNA-dependent RNA polymerase in the NS5b part of the HCV genome was amplified. PCR amplifications were conducted in a BioRad DNA Engine using Quantitect SYBR Green PCR mix. Purified PCR products expressively sequenced with the ABI PRISM 310 Genetic Analyzer appliance utilizing DYEnamic ET Terminator Cycle Sequencing Kit. The most prevalent genotype was type 1b (90.4%) in our study. In the other types, type 3 and 4 were detected. We believe that distribution of HCV genotype in this region should be followed strictly due to difference from reports, which reported other parts of Turkey.
Highlights
Hepatitis C virus (HCV) has infected about 170-200 million people worldwide, and only half of patients treated with the actual accepted therapies achieve a sustained viral reply [1,2]
At least six major HCV genotypes and hundreds of subtypes have been determined based on nucleotide distinctness within core worldwide so far [7,8,9]
The knowledge of genotypes in chronic hepatitis C is crucial for the decision of the therapeutic regimen and for the therapeutic outcome; genotypes 2 and 3 are curable in more than 80%, whereas genotypes 1 and 4 are curable in only 40-50% of circumstance [5]
Summary
Hepatitis C virus (HCV) has infected about 170-200 million people worldwide, and only half of patients treated with the actual accepted therapies achieve a sustained viral reply [1,2]. The HCV accounts for 20% of acute and 70% of chronic cases of hepatitis [3]. HCV emerges abundant genetic heterogeneity, as a result of aggregation of mutations during replication. Accumulation of nucleotide substitution in the HCV genome results in alteration and evolution into distinct genotypes, subtypes [1]. At least six major HCV genotypes and hundreds of subtypes have been determined based on nucleotide distinctness within core worldwide so far [7,8,9]. Some seem to have spread worldwide (genotypes 1a, 1b, 2a, 2c, 3a), while others have been detected in more restricted areas only (genotypes 4, 5a, and 6a) [10]
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