The differences in the levels of interleukin 1 beta (IL-1ß) and tumor necrosis factor alpha (TNF-Α) based on the storage of thrombocyte concentrates

Abstract

Thrombocyte concentrate (TC) transfusion plays an important role in preventing bleeding in patients with severe thrombocytopenia. Febrile non hemolytic transfusion reaction (FNHTR) may occur after TC administration. IL-1ß and TNF-α cytokines released by thrombocytes and leucocytes during TC storage play important roles in the occurrence of FNHTR after TC transfusion. The purpose of this study was to analyze changes in levels of  IL-1ß and TNF-α on the duration of TC storage. This was an observational analytical research with time series design carried out at the Clinical Pathology Laboratory and Blood Bank of the Dr. Soetomo Hospital Surabaya in September - October 2019. IL-1ß and TNF -α levels in 20 bags of Thrombocyte Concentrate blood components derived from Platelet Rich Plasma during storage for day 1, day 3 and day 5 were measured using ELISA Sandwich method. The statistical analysis was performed using the Subject Same Variant Test or Friedman Test. The results showed no significant differences in the levels of IL-1ß and TNF-α based on the storage duration of TCs on day 1, 3 and 5, p = 0.262 and p = 0.534 respectively. There was a significant difference of IL-1ß levels between day 1 and day 3 (p=0.032).