Abstract
The intracellular and extracellular [H+] of a series of umbilical cord blood samples have been measured by lysis of a packed red cell mass. Samples of adult and fetal whole blood were equilibrated for 30 minutes with varying CO2 tensions in tonometers. It was found that the intracellular [H+] of fetal erythrocytes was identical to that of adult erythrocytes at any given extracellular [H+]. Hence, the difference in the oxygen dissociation curves of adult and fetal whole blood cannot be ascribed to differences in their intracellular [H+]. The intracellular and extracellular [H+] of a series of umbilical cord blood samples have been measured by lysis of a packed red cell mass. Samples of adult and fetal whole blood were equilibrated for 30 minutes with varying CO2 tensions in tonometers. It was found that the intracellular [H+] of fetal erythrocytes was identical to that of adult erythrocytes at any given extracellular [H+]. Hence, the difference in the oxygen dissociation curves of adult and fetal whole blood cannot be ascribed to differences in their intracellular [H+].
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