The Diagnostic Value of the Second Generation Anti-CCP Test in Rheumatoid Arthritis

Dejan Spasovski,Baskim Osmani,Snežana Percinkova,Ljubinka Rajčevska,Todor Gruev,Kočo Cakalaroski,Jordan Calovski,Nada Marina

doi:10.2478/v10011-007-0043-3

Abstract

The Diagnostic Value of the Second Generation Anti-CCP Test in Rheumatoid ArthritisThe purpose of this research was to compare the diagnostic values of laboratory variables, to present quantitative evaluations of the diagnostic test with reference to sensitivity, and specificity, the predictive value of the positive and negative test and precision of the test for anti-cyclic citrullinated peptide (anti-CCP 2) antibodies, rheumatoid factor (RF), C-reactive protein (CRP), DAS 28 index, in early diagnosis of untreated rheumatoid arthritis (RA). Using the ELISA technology of DIA-STAT™Anti-CCP (Axis-Shield Diagnostics), the serum has been examined in 70 participants (35 RA who were not treated, 35 healthy controls). RF was defined with the test for agglutination (Latex RF test) in the same participants. In 23 of the 35 examined patients with RA, we found presence of anti-CCP 2 antibodies (sensitivity of the test 65.71%), while RF appeared in 17 patients (sensitivity of the test 48.57%). Twelve patients were anti-CCP 2 and RF positive, 11 were anti-CCP 2 positive but RF negative. Five patients were anti-CCP 2 negative and RF positive. Out of 18 RF negative patients, 11 were anti-CCP 2 positive. In 17 RF positive patients, anti-CCP 2 antibodies were positive in 12 patients. In the healthy control group, 1 patient was anti-CCP 2 positive, while 2 patients were RF positive. Anti-CCP 2 antibodies have higher sensitivity and specificity than RF in RA.

Highlights

Rheumatoid arthritis (RA) is a systemic autoimmune disease that is multi-functional in origin and is characterised by the inflammation of the membrane lining joints
Summary: The purpose of this research was to compare the diagnostic values of laboratory variables, to present quantitative evaluations of the diagnostic test with reference to sensitivity, and specificity, the predictive value of the positive and negative test and precision of the test for anti-cyclic citrullinated peptide antibodies, rheumatoid factor (RF), C-reactive protein (CRP), DAS 28 index, in early diagnosis of untreated rheumatoid arthritis (RA)
Using the enzyme-linked immunosorbent assay (ELISA) technology of DIA-STATTM Anti-cyclic citrullinated peptide (CCP) (Axis–Shield Diagnostics), the serum has been examined in 70 participants (35 RA who were not treated, 35 healthy controls)

Summary

Introduction

Rheumatoid arthritis (RA) is a systemic autoimmune disease that is multi-functional in origin and is characterised by the inflammation of the membrane lining joints. The disease spreads from small to large joints, with the greatest damage in the early phase [1]. The IgM class is the most common and is found in 60–80% of RA patients. RF is not specific for RA, as it is often present in healthy individuals and patients with other autoimmune diseases and chronic infections [2]. It is reported that up to 30% of SLE patients with no evidence of RA are RF positive [3]. A positive RF is considered an important predictor of outcome in RA

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