The Diagnostic Significance of PDGF, EphA7, CCR5, and CCL5 Levels in Colorectal Cancer.

Üçüncü Üçüncü,Sarı Sarı,Bademler Bademler,Serilmez Serilmez,Karabulut Karabulut

doi:10.3390/biom9090464

Üçüncü Üçüncü, Sarı Sarı + Show 3 more

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https://doi.org/10.3390/biom9090464

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Journal: Biomolecules	Publication Date: Sep 9, 2019
Citations: 26	License type: CC BY 4.0

Affiliation: Istanbul University

Abstract

In this study, we compared the levels of C-C chemokine receptor type 5 (CCR5), C-C motif chemokine ligand 5 (CCL5), platelet-derived growth factor (PDGF), and EphrinA7 (EphA7) in patients with colorectal carcinoma and healthy controls in order to investigate the significance and usability of these potential biomarkers in early diagnosis of colorectal cancer. The study included 70 colorectal carcinoma patients and 40 healthy individuals. The CCR5, CCL5, PDGF, and EphA7 levels were measured using ELISA in blood samples. PDGF-BB, EphA7, CCR5, and CCL5 levels of the patients with colorectal carcinoma were significantly higher compared to the control group (p < 0.001 for each comparison). Our logistic regression analysis (the area under the curve was 0.958) supports the notion that PDGF-BB, EphA7, and CCL5 are potential biomarkers for the diagnosis of colon cancer. The sensitivity, specificity, and positive and negative predictive values were found to be 87.9%, 87.5%, 92.1%, and 81.4%, respectively. To our knowledge, this is the first study that investigates the relationship between colorectal carcinoma and the four biomarkers CCL5, CCR5, PDGF, and EphA7. The significantly elevated levels of all these parameters in the patient group compared to the healthy controls indicate that they can be used for the early diagnosis of colorectal carcinoma.

Highlights

Colorectal carcinoma (CRC) is the most common gastrointestinal cancers worldwide and one of the most common four cancers in both sexes in Turkey
The significantly elevated levels of all these parameters in the patient group compared to the healthy controls indicate that they can be used for the early diagnosis of colorectal carcinoma
This is the first study that has investigated whether the cytokines/chemokines C motif chemokine ligand 5 (CCL5), chemokine receptor type 5 (CCR5), platelet-derived growth factor (PDGF), and EphA7 have significant value for early diagnosis of colorectal carcinomas

Summary

Introduction

Colorectal carcinoma (CRC) is the most common gastrointestinal cancers worldwide and one of the most common four cancers in both sexes in Turkey. Studies focused on the early diagnosis of CRCs continue to assess different indicators [1,2,3,4]. The genetic defects that lead to colorectal cancer development cause the release of various molecules, inducing the growth, proliferation, and death of cancerous and normal cells [5]. Fibroblasts, extracellular matrix components, growth factors, and cytokines/chemokines all play important roles during tumor progression and the metastasis of CRC [6,7]. CRC is considered a single type of tumor tissue, it involves a heterogeneous group of pathologies

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