The diagnostic combination of serum circulating miR-488 and lncRNA AC018761 as biomarkers for hypopharyngeal squamous cell carcinoma (HPSCC)

Abstract

ObjectiveThe search for rapid and effective early diagnostic markers of hypotharyngeal carcinoma becomes particularly critical and important. Therefore, we explored the value of circulating expression of non-coding RNAs for the diagnosis of early hypotharyngeal cancer. MethodsAfter screening HPSCC-related research data in the NCBI-GEO database, the practical report extraction language (Perl) was used to give genes different biotype attributes. The R language limma package was utilized to analyze the differential expression of lncRNAs and mRNAs, respectively.The quantitative RT-PCR (Reverse transcription-polymerase chain reaction) analysis were performed to determine the plasma expression levels of non-coding RNAs ResultsCompared with the control groups, the differential expression of 137 lncRNAs and 297 mRNAs in the plasma of HPSCC patients was statistically significant. There were 32 differentially expressed lncRNAs, 19 differentially expressed mRNAs. The score of LncRNA AC018761 + miR-488 was AUC = 0.846, the best cut-off value was ≥ 40 points, the sensitivity was 74.58%, and the specificity was 79.88%. ConclusionOur results indicated that the over-expression of lncRNA-AC018761 and miR-488 were significantly up-regulated in serum of patients with HPSCC. Therefore, lncRNA-AC018761 and miR-488 maybe the non-invasive independent predictor for early HPSCC with high sensitivity and specificity.