The diagnosis value of enzyme-linked immunespotassay test the IFNγ in lymphoid tuberculosis

Abstract

Objective To evaluate the clinical value of myeolymphoid tuberculosis specific IFNγ assay by the enzyme-linked immunespot (Elispot) for diagnoses of lymphoid tuberculosis. Methods Collect the clinical data of 89 patients who had been diagnosed and treated as lymphoid tuberculosis by the Third People’s Hospital of Shenzhen during December 2011 and December 2013. Compare and analyze the positive detection rate in lymphoid tuberculosis diagnosis by different methods including Elispot detection, mycobacterium tuberculosis cultivation, microscopic PCR detection and pathologic examination. Results Among the 89 patients, the positive detection rate of lymphoid tuberculosis by Elispot was 79.78% (71/89) , 39.29% (11/28) by Mycobacterium tuberculosis cultivation, 53.57% (30/56) by PCR, and 84.51% (60/71) by pathology diagnosis. The positive rate of Elispot was significantly higher than Mycobacterium tuberculosis cultivation and PCR, of which the differences were statistically significant (χ2=16.655, 11.166, P both<0.05) . The positive rate were 89.29% (25/28) , 82.14% (46/56) when Elispot was combined with mycobacterium tuberculosis cultivation and PCR. Combining with Elispot, tuberculosis bacterium cultivation and polymerase chain reaction (PCR) , the positive rate could reach up to 91.67% (22/24) . Conclusions Elispot was proved to be a very useful method in diagnosis of lymphoid tuberculosis, which improves the positive rate a lot when combining with other common clinical detection method. Key words: Tuberculosis; Diagnosis; Enzyme-linked immunespot assay; Interferon