The developmental morphology of Torpedo marmorata: electric organ--electrogenic phase.

Abstract

The electrogenic developmental phase of the electric organ of Torpedo marmorata begins at 40 mm of embryo length and is characterized by a horizontal flattening of the vertically orientated myotubes. The first sign of this process is a rounding up of the ventral poles of the myotubes and a disassembly of the myofibrils located therein. Occurring concomitantly with this is a migration of the nuclei to the cell center which results in a horizontal plane of nuclei. Filament bundles are then found within the ventral cytoplasm often projecting upwards from the ventral plasma membrane. The filaments of the bundles are dimensionally similar to the myofilaments of muscle and it is suggested that the bundles play a role in cellular transformation. In contrast the dorsal pole of the cell appears to be integrated "passively" with the final cell shape as no morphological correlates of a retraction process have been found. A canalicular system, composed of a complex network of irregular tubules and vacuoles, appears just below the dorsal plasma membrane characterizing this region of the cell. A mononucleated satellite cell population lies in close proximity to the dorsal surface of the differentiating cell and fusion between the two cell types occurs throughout development. Cell shape transformation is complete by 55 mm of embryo length and the intercolumnar nerves begin to invade the interelectrocyte space. The ingrowing neurites preferentially course along the ventral electrocyte surface establishing junctions similar to motor endplates.