Abstract

In the all-rod retina of the adult mouse, synaptic contacts in the outer plexiform layer occur between photoreceptor terminals and dendritic processes of horizontal and bipolar cells. The postnatal development of the outer plexiform layer has been studied by light and electron microscopy. Following decapitation and enucleation of the eyes, samples of retinae from DBA mice (newborn to fourteen days) were removed and fixed by immersion in solutions of buffered glutaraldehyde and paraformaldehyde. The tissue was postfixed in osmium tetroxide, dehydrated and embedded in araldite. Sections were stained with uranyl acetate and lead citrate.With light microscopy, the outer plexiform layer first appears in the central retina as a gap within the neuroblastic layer on the fifth postnatal day. The separation is completed by the seventh postnatal day and appears mature by the fourteenth postnatal day.

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