Abstract
A refined analytical method has been developed for the determination of the isotope ratio of oxygenated compounds in essential oils, using high-resolution gas chromatography-mass spectrometry (HRGC-MS), has been developed. Thirty-three samples of yuzu fruits from different production areas in Japan and South Korea were collected and prepared for cold-pressed peel oils. The oils were analysed by HRGC-MS for linalool concentration and isotope ratio based on the peak intensities of (M+2)+, (M+1)+ and M+ ions. A significantly lower isotope ratio m/z 156/154 and m/z 155/154 were observed for the yuzu essential oil from Goheung and Kyoto areas. Statistical analysis showed the isotope ratio of linalool to be useful in the discrimination of yuzu essential oils from different geographical origins.
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