Abstract
The development of a stationary phase material for high-performance liquid chromatography based on a surface of silica hydride as opposed to silanols on ordinary silica is discussed including synthetic approaches, characterization, and applications. There are several synthetic approaches available to create a silica hydride surface. Modification of the Si–H moiety on the silica surface can be accomplished through the use of a hydrosilation reaction. Both the intermediate silica hydride and the material modified with an organic moiety can be characterized by a number of spectroscopic as well as a variety of other methods. Further insights into the retention mechanism are provided through chromatographic measurements. The ultimate utility of any chromatographic stationary phase material is determined by its success in solving challenging analytical problems. A broad range of applications is reviewed to illustrate the versatility and usefulness of silica hydride-based stationary phases.
Highlights
The fundamental properties of silica as a support material for high performance liquid chromatography were described by Unger [1] quite some time ago
The unique feature of the analysis shown for is that this hydrophilic compound is strongly immunosuppressant methotrexate
The unique feature of the analysis shown is that this hydrophilic compound is strongly possible on an ordinary silica C18 stationary phase that only retains compounds in the reversed-phase a silicaphase hydride material modified with an octadecyl moiety
Summary
The fundamental properties of silica as a support material for high performance liquid chromatography were described by Unger [1] quite some time ago. In the case of monomeric bonding, an organosilane compound with one reactive group reacts with the silanol on the silica surface to produce a single point of attachment via silicon-oxygen-silicon-carbon bonds. In the case of monomeric bonding, an organosilane compound with one reactive group reacts on the silica surface to produce a single point of attachment via silicon-oxygen of 15 silicon-carbon bonds. A drawback of the organosilanization approach is the Si–O–Si–C linkage at the surface between the silica support material and the bonded organic moiety. A goal attachment is subject to hydrolysis while at higher pH the support silica is prone to a dissolution for improvement of stationary phase stability is to fundamentally alter this bonding reaction and to process.
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