Abstract
The study was performed at the Department of Clinical Pathophysiology, Topographical Anatomy and Operative Surgery at Kharkiv Medical Academy of Postgraduate Education as a part of research project "Radiotoxins' pathochemical mechanisms and methods of early diagnostics and correction", state registration No. 0117U000589.
 We studied the subtoxic effect of small doses of sodium fluoride on the activity of microsomal hepatocytes on 30 Wistar rats' populations in subacute experiment. The intensity of lipid peroxidation (LPO) in liver of rats which were administered sodium fluoride orally for a long time at doses of 1/10 and 1/100 LD50, was evaluated by the content of its molecular products - diene conjugates (DC), and MDA-reagents Schiff bases. We found that oral administration of sodium fluoride to rats at doses of 1/10 and 1/100 LD50 promotes a statistically significant increase (r≤0,002) relative to the control group of animals in DC content during the entire period of observation. At a dose 1/10 LD50 we observed the most significant increase in this indicator on the 10th day of the experiment - at 265%, and at a dose 1/100 LD50 – on the 20th day an average of 234%. In rats’ liver by the action of subtoxical dose of sodium fluoride at a dose of 1/10 LD50, starting from the 20th day, we detected a gradual increase (r≤0.001) of TBA-reagents relative to control - for 27, 41, 78, 133%. Secondary end products and lipid peroxidation, which are defined under the long-term of sodium fluoride, somehow contribute to the disruption of the microstructure of hepatocytes membranes, their permeability, reduce their division, regeneration and inhibition of mitochondrial respiratory chain enzymes and microsomal monooxygenase system.
Highlights
The main biological role of fluoride and its compounds consists in bone formation, formation of dentin, enamel, preventing the development of senile osteoporosis
The aim of this study is to investigate the state of free radical processes and lipid peroxidation in the liver of rats with chronic fluoride intoxication
Rats were subjected to oral seed probe using aqueous solutions of sodium fluoride (FS) once daily for 60 days at doses of 1/10, 1/100 and 1/1000 LD50, which were under 20 mg/kg, 2 mg/kg and 0.2 mg/kg body weight (FS average lethal dose for rats received orally, is 200 mg/kg)
Summary
The main biological role of fluoride and its compounds consists in bone formation, formation of dentin, enamel, preventing the development of senile osteoporosis. High concentrations of fluoride stimulate lipid peroxidation and inhibit antioxidant defense system. It belongs to the elements of the first class of danger - especially hazardous chemicals. Prolonged excessive intake of fluoride compounds in the composition of the body can cause pathological condition - fluorosis. Despite the significant content of fluoride in different tissues of the human body, its physiological role so far has not been clarified. The processes of free radical oxidation constitute an important part of nonspecific metabolic component of adaptation to the effects of stress factors, including chemi-
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