Abstract
β-Conglycinin is an important storage protein in soybean, which can potentially cause food allergies in human. In this study, a sensitive mouse monoclonal antibody (3D11 mAb) with a high affinity was prepared, and sandwich lateral flow immunochromatographic detection strips were developed for the rapid detections of the soybean allergen β-conglycinin. The 3D11 mAb was combined with a rabbit polyclonal antibody in order to establish strips. The titer of 3D11 mAb was 1:2.56×105. The affinity constant of the 3D11 mAb was 9.6×109. The lowest detection limit with the naked eye of the double antibody sandwich strips was 1μg/mL. In addition, chemical molecules p-aminothiophenol with colloidal gold were used as Raman enhancement signals in order to achieve quantitative detections of the β-conglycinin. It was determined in this study that the practical working range of the β-conglycinin concentrations was between 160ng/mL and 100μg/mL with the developed assay.
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