The Development of Immunoenzyme Monoclonal Test Systems for Detection of Tularemia, Glanders, Melioidosis and Anthrax Agents

Abstract

The identification of causative agents of dangerous infectious diseases of bacterial origin is one of the main tasks of the biological protection of troops and the civilian population of the Russian Federation. This article is dedicated to the problem of the development of immunoenzyme monoclonal test system for detection of tularemia, glanders, melioidosis and anthrax agents. In order to achieve the above-mentioned goal, the hybridomas-producers of monoclonal antibodies to antigenic determinants of pathogens of tularemia, glanders, melioidosis and anthrax have been prepared. The pairs of monoclonal antibodies, the application of which in the composition of sensitin and immunoperoxidase conjugate provide maximum sensivity and specificity of enzyme immunoassay test, have been selected and the immunoenzyme monoclonal test systems for detection of tularemia, glanders, melioidosis and anthrax agents have been developed based on the above mentioned monoclonal antibodies. These developed test-systems can help to identify the pathogens of dangerous infectious diseases in microbial cultures, in probes taken from sick and dead people and animals and from environmental probes.