Abstract
Monoclonal and polyclonal antibodies play a key role in medicine as well as in analytical biotechnology. The methods for purification of antibodies are developed fast. Hydrophobic charge induction chromatography (HCIC) is a recently developed method for protein separation based on the use of dual-mode ligands. They are designed in such a way so as to combine a molecular interaction supported by a mild hydrophobic association effect in the absence of salts. When pH is changed, the resulig and becomes ionically chargedre sulting into the desorption of the protein. HCIC shows specific advantages (such as high degree of purity and recovery) and is used in several labs and manufactures for antibody production. This paper reviewed the basic principle, development and application of HCIC. Key words: Hydrophobic charge induction chromatography; Antibody purification; Ligands
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