The development of an immunoblotting assay for the quantification of liver fatty acid-binding protein during embryonic and early posthatch development of turkeys (Meleagridis gallopavo)

Abstract

Turkey (Meleagridis gallopavo) liver cytosolic fatty acid binding protein (FABP) was purified and used as a standard for quantification. An immunoblotting procedure was developed to study the ontogeny of liver cytosolic FABP during embryonic and early posthatch development in turkey poults. Liver FABP activity was also determined indirectly through the use of gel filtration chromatography followed by a ligand-binding assay. The specific activity of liver FABP (ng/mg of cytosolic protein) increased with length of incubation, peaking initially at Day 22, declining between Days 22 and 25, and increasing again from hatch (Day 28) to 6 d posthatch. The specific activity of liver FABP increased 12-fold between Day 13 of incubation and 6 d posthatch compared with total activity, which increased from 946 to 1.01 × 10(6) ng/liver during the same period, a 1,067-fold increase. The results from both analytical procedures were similar, suggesting that the immunoblot method could be used to quantify liver FABP concentrations. The observed increases in FABP activity throughout the embryonic period and first days after hatching paralleled increases in liver lipid concentration. Therefore, liver FABP may be associated with hepatocyte fatty acid transport and metabolism during the latter stages of incubation and early posthatch period.