Abstract
This article describes the optimisation of a solid-phase immunoenzyme assay (ELISPOT) for the detection of individual antibody-secreting cells in fish. Its use to detect specific and total antibody-secreting cells in lab Limanda limanda is shown and the relative size of the specific B cell response in relation to the total number of antibody-secreting cells and serum antibody is given. Its potential application to a variety of areas of fish immunology is discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
