Abstract
In both plant and animal cells, membrane is continuously being taken from (endocytosis) and added to (exocytosis) the plasma membrane via vesicles. Presently it is believed that the cell regulates these two events by balancing one with the other. In plants no direct proof of this theory exists, due to the lack of proper markers for labelling the incoming vesicles. These markers have to be small enough to traverse the cell wall, yet large enough to be visualised in the electron microscope. Gold particles with a mean diameter of 3.5nm have been developed and used as a marker on plant cells ( Morinda citrifolia) cultured in a liquid medium. Initial results showed that the gold marker penetrated the cell wall, indicating that the wall is more permeable than previously thought. Gold particles were located in the cytoplasm, indicating uptake into the cell via endocytosis.
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