The Development of a Genetic Linkage Map of Redcurrant (Ribes rubrum L.) by Means of SSR and SNP DNA Markers

Abstract

This work was aimed at compiling the world’s first linkage map of redcurrant with the use of microsatellite and SNP DNA markers. As a result of mapping, eight linkage groups with an LOD level of 10 were constructed, which included 299 DNA markers, including 12 microsatellite and 287 SNP markers; the total length of all linkage groups was 741 cM; the average was 93 cM. In seven of the eight linkage groups obtained, at least one microsatellite DNA marker was mapped. Most of the microsatellite loci localized on the redcurrant map correspond to their position on the blackcurrant linkage groups (loci e3-B02, g1-A01, and g1-L12 are located on the same linkage group no. 5; loci g2-J08, g2-B20, e1-O21, and e1-O01 on the resulting map are each in a separate linkage group 2, 3, 4, and 6, respectively), with the exception of the loci mapped at blackcurrant on linkage group 1, which in our work were unmapped. The g2-J11 locus in our studies amplifies fragments in two ranges, which suggests its duplication in the genome of the studied samples. This locus was mapped in two linkage groups of redcurrant. Two new, not previously mapped and unpublished microsatellite loci were also localized on linkage map of redcurrant. In the future, it is planned to localize economically useful traits in the redcurrant genome.