Abstract
Barred galaxias, Galaxias fuscus, is an endangered freshwater fish confined to headwaters of mountain streams in the Goulburn River system in central Victoria, Australia. Next generation DNA sequencing was used to develop a suite of microsatellite markers that can be used for future population genetic assessments of G. fuscus. A total of 24 polymorphic loci were identified and 12 characterized using 30 individuals from a single sample locale. We observed low to moderate genetic variation across most loci (mean number of alleles per locus = 2.58; mean expected heterozygosity = 0.37) with no evidence of individual loci deviating significantly from Hardy–Weinberg equilibrium. Marker independence was confirmed with tests for linkage disequilibrium, and analyses indicated no evidence of null alleles across loci. The 12 markers characterized in the present study provide a valuable resource for future population genetic assessments and management of G. fuscus in Australia.
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