Abstract
The status of susceptible mosquito is important for monitoring and managing insecticide resistance in field population. The purpose of this study is to profile the differential expression level of enzymatic activities of metabolic enzymes exhibited in the mosquito species of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus from the susceptible strain. The fourth instar larvae of each strain were subjected to biochemical assay. The total protein content and enzymatic activities of Glutathione S-transferase (GST), α-esterase (α-est), β-esterase (β-est) Cytochrome P450 (Cyt P450) and Acetylcholinesterase (AChE) from each mosquito strains were elucidated. Significant difference (P<0.05) was detected between the total protein content between all species of susceptible strain mosquitoes. One-way ANOVA showed that the specific enzymatic activities of GST, α-est and Cyt P450 of all test mosquitoes were significantly different upon comparison with each species (P<0.05). The mean of enzymatic activities of insensitive AChE showed no significant difference upon comparison with each other (P>0.05). The enzymatic activities of β-est shows no significant difference between Ae. aegypti and Cx. quinquefasciatus mosquitoes (P>0.05) but the enzymatic activity of β-est in Ae. albopictus manifested significant difference upon comparison with the enzymatic activities of the other two test species (P<0.05). The results obtained may provide more information about the enzymatic activities of metabolic enzymes in Ae. aegypti, Ae. albopictus and Cx. quinquefasciatus mosquitoes which might be beneficial for public sector for the application of proper vector control measures.
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More From: IOP Conference Series: Earth and Environmental Science
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