Abstract
AN ACCURATE method for the determination of uric acid in poultry excreta is of great importance in protein metabolism studies, for uric acid forms the most important nitrogenous constituent in avian urine. Uric acid, as precipitated from an alkaline extract of poultry excreta, is admixed with other non-uric acid reducing substances which also react with strong oxidizing substances such as potassium permanganate, ceric sulphate, etc., generally used for the titration of uric acid. Bose (1943) reported a simple and accurate iodimetric method for the estimation of uric acid in poultry excreta. With the iodimetric method, the non-uric acid substances with higher oxidation potential do not interfere in the titration.In the present investigation, another method for the determination of uric acid in poultry excreta has been developed by taking advantage of the specific action of uricase on uric acid and this method has been compared with the iodimetric method (Bose, .
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