Abstract
A quantitative procedure has been devised for the isolation of hydroxyproline from hydrolyzed urine or collagen samples by chromatography on a column of Dowex 50W. When combined with the colorimetric procedure described here, duplicate analyses agree to within 2.0%, and mean recoveries of 98.3% are obtained. The application of this method to the determination of hydroxyproline in urine and the measurement of specific activities in bone extracts is described. Two shorter methods, not involving chromatography, are also described. These procedures give fairly precise results, but yield somewhat lower recoveries and lower final values than the chromatographic procedure. The results suggest that a substance is present in urine and collagen which reduces the final color yield by the chloramine T-Ehrlich's reagent colorimetric procedure. This substance is removed by chromatography on Dowex 50W, and may be an amino acid. One of the shorter procedures, although yielding values which are on the average 19% low, may be useful for the rapid, routine measurement of hydroxyproline in clinical urine specimens.
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