Abstract
A rapid and accurate method for the direct determination of total and ester cholesterol in whole blood, serum or plasma is presented. After petroleum ether extraction of the cholesterol, an aliquot of the extract after solvent removal is treated with a digitonin solution and after a short precipitation time the cholesteryl esters are selectively extracted. A FeCl3-H2SO4 reagent is used for colour development. It has been shown that the cholesteryl esters produce the same optital density with this colour reagent as an equimolecular amount of free cholesterol. The method gives good reproducibility, recovery of added free and ester cholesterol is quantitative and there is good agreement with the method of sperry and webb.
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