The Determination of the Partial 28S Ribosomal DNA Sequences and Rapid Detection of Phellinus linteus and Related species

Abstract

Species of Phellinus were known to harmful fungi causing white pocket rot and severe plant disease such as canker or heart- rot in living trees in the West, but some species have been used to traditional medicines in the Orient for a long time. In this study the partial D1-D2 nucleotide sequences of 28S ribosomal DNA from 13 Phellinus strains were determined and compared with the sequences of 21 strains obtained from GenBank database. According to the neighbor-joining (NJ) method comparing the sequence data the phylogenetic tree was constructed. The phylogenetic tree displayed the presence of four groups. Group I includes P. ferreus, P gilvus and P johnsonianus, Group II contains P laevigatus, P. conchatus and P. trem- ulae, Group III possesses P. linteus, P. weirianus, P. baumii, P. rhabarbarinus and P igniarius, and Group IV comprises P. pini, P chrysoloma. P linteus and P baumii, which were used mainly in traditional medicine, belong to the same group, but exactly speaking both were split into two different subgroups. To detect P linteus only, we developed the PCR primer, D12HR. The primer showed the specific amplification of P linteus, which is permitted to medicinal mushroom in the East. The results make a potential to be incorporated in a PCR identification system that could be used for the rapid identification of this species from its related species, P linteus especially.