The determination of the molecular size of peptides and proteins by chromatography on Bio Gel P-100 columns in phenol-acetic acid-water (1:1:1, w/v/v) solvent mixture

Abstract

1. 1.|The results of our studies of molecular sieve chromatography on a Bio Gel P-100 column in phenol-acetic acid-water (1:1:1; w/v/v) of a number of well-characterized proteins, polypeptides and other compounds of known molecular weight indicated the existence of the following empirical relationship between the elution volume and the log of the molecular weight: log 10 mol. wt. = [5.13 −(0.53 ± 0.013) x (V e/V o)] ± 0.059 Of the nearly thirty individual compounds studied cytochrome c and insulin A chain were found to deviate from the above equation. 2. 2.|Application of this and similar chromatographic techniques in other dissociating solvent systems for the investigation of insoluble and structural proteins was discussed.