Abstract
A method is described for the determination of fatty acid patterns of individual phospholipids ( viz. lecithin, lysolecithin and sphingomyelin) and neutral lipids ( viz. sterol esters, free fatty acids and triglycerides) of serum by gas-liquid chromatography, after their separation by thin-layer chromatography. Replicate determinations indicate that the method gives highly reproducible results, and there is no evidence of loss of unsaturated fatty acids by oxidation. In a comparison between thin-layer and column chromatographic methods, values for the fatty acid composition of cholesterol esters, triglycerides and free fatty acids were in good agreement. With phospholipids agreement for values of lecithin and lysolecithin was moderately good but with sphingomyelin agreement was poor. The thin-layer method has the advantage of being much faster and requiring smaller quantities of lipid for analysis.
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