Abstract
Quantitative studies of the release of NO in vivo are difficult due to its short half-life. Consequently, most investigators have assayed nitrite and/or nitrate because these are relatively stable markers of NO production. Although various methods have been developed, all are limited to some extent by the degree of sample handling required. To overcome this we have developed a simple capillary electrophoresis method which requires very little sample handling and can measure both nitrite and nitrate in a single short run time. The method is based on the principles of capillary ion analysis and offers high sensitivity, good precision, and high throughput. The limit of detection is approximately 200 nM for nitrite and 300 nM for nitrate. Regression analyses for both anions routinely give values of greater than 0.99. Basal values obtained with this method are in good agreement with literature values.
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