Abstract
The reaction of nucleic acids with cysteine in sulfuric acid and the use of TCA for their extraction from tissue have been reinvestigated. Modified procedures have been developed for the determination of DNA and RNA in certain animal tissues and tissue fractions. The interference of DNA in the determination of RNA was eliminated by exploiting the instability of DNA in hot TCA solutions. Complete extraction of nucleic acids was accomplished by two separate extractions with 10% TCA, 8 min. for DNA and 40 min. for RNA. Among samples ranging from 0.1 to 4% DNA phosphorus and from 0.1 to 0.6% RNA phosphorus, the average accuracy was approximately ±0.05% phosphorus in either procedure.
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