Abstract
Using a combined gas chromatographic-mass spectrometric-selected ion recording technique it has been possible to achieve detection, identification, and quantitation of nicotine in biological fluids in amounts as low as 25 pg. Employing the deuterated variant nicotine-5′,5′- d 2 both as an internal standard and as a carrier in an inverse isotope dilution method, an accurate, sensitive, and specific method for picogram level quantitation of nicotine is obtained. Careful precautions were necessary to prevent positive “blank” values at low levels due to extraneous nicotine, which were found to arise both from the solvents used in the extractions and from airborne sources.
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