Abstract
1. Different methods for determining lecithin: cholesterol acyltransferase (LCATase) activity in plasma of Ameiva ameiva were investigated. 2. In vivo labelling of plasma unesterified cholesterol (UC) following administration of dl-[2-3H]mevalonate was poor and could not be used as the basis for radiochemical assay of LCATase. 3. Determination of LCATase rates using heated plasma-4[-14C]cholesterol substrates gave results which varied with the substrate plasma used. 4. LCATase rates measured enzymically as the decrease in UC levels after plasma incubation gave results similar to, and which correlated significantly with, the rates measured radiochemically using heated rat plasma.
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