Abstract
A simple method for the determination of γ-aminobutyric acid in brain tissue is described. Two columns of ion-exchange resin are used. An extract of rat brain is first passed through Amberlite CG-50, which removes basic amino acids and amines. The effluent from this column is then passed through a column of Dowex 50 buffered at pH 3.1. Elution from this column with pH 3.1 buffer removes other interfering amino acids from the resin. Good recoveries of GABA added alone, in a mixture of amino acids, or mixed with a true brain extract were obtained. Values of the GABA content of brain obtained by this method agree well with those reported in the literature.
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