Abstract
Interference by various members of the vitamin B complax in the reineckate method for the determination of choline may be overcome by precipitation of choline at controlled pH levels. Thiamine, nicotinic acid, nicotinamide, and pyridoxine, which ordinarily produce precipitates with ammonium reineckate reagent, are not preapitated in alkaline solution from which choline may be separated as the insoluble reineckate. This is utilized as a basis for separation and the subsequent colorimetric determination of choline. Data are given showing the effect of increasing pH on the precipitation of the interfering B vitamins and on choline by ammonium reineckate reagent, and on the recovery of choline from mtxtures of the various vitamins of the B complex.
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