Abstract
The method consists in a direct precipitation of the calcium without removal of the blood porteins. The only reagents necessary are ammonium chloride (to prevent the precipitation of magnesium), and ammonium oxalate, both adjusted approximately to PH 7.4.Blood.—Citrated blood (5 c.c. in a 50 c.c. centrifuge tube), is hemolyzed by the addition of four volumes (20 c.c.) of warm water and after standing 15 to 20 minutes is centrifuged to remove the stroma. An aliquot (20 c.c.) of the clear, red liquid is transferred to a 50 c.c. centrifuge tube. One c.c. of 5 per cent. ammonium chloride is added and, after mixing, 3 c.c. of 3 per cent. ammonium oxalate are added. The oxalate should be added slowly and the contents of the tube must be well mixed. After standing at least 16 hours (the much shorter time recently reported by de Waard1 results in an incomplete precipitation of the calcium), the mixture is centrifuged and the supernatant liquid siphoned off. The tube is washed by the addition of 25-30 c.c. of cold...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
