Abstract
A range of biogenic amines were measured in the heads from four strains of Drosophila melanogaster. Quantitation was carried out using gas chromatography—negative ion chemical ionization mass spectrometry (GC—NICIMS) with stable isotope dilution. The principal amines detected in the heads were dopamine, noradrenaline and 5 HT with small amounts of p- and m-tyramine; p-octopamine could not be detected in samples of 25 heads with a limit of detection of 10 pg per sample. In addition to conventional neurotransmitters or putative neurotransmitters the amines 5- and 6-hydroxydopamine were detected in the heads in substantial amounts.
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