Abstract
There were analyzed the conditions under which the basal glycemia and/or glucose tolerance curve is not contaminated by stress induced changes in glycide metabolism. When the basal glycemia was monitored, blood was sampled to heparinized capillaries from retrobulbar plexus under the light ether anaesthesia or by decapitation without narcosis. The animal represented the control for itself. No differences was found in basal glycemia under the two mentioned blood sampling. In the second series of experiments glycemia was monitored in the time schedule which is used in glucose tolerance test, i.e., blood sampling was performed 30, 60, 120 and 180 min after the first sampling from retrobulbar plexus either under light ether anaesthesia (in 14 h starvated rats or in rats with free access to diet) or under Nembutal anaesthesia (in 14 h starvated rats). No differences were found in glycemia when two types of narcosis is compared. No signs of augmentation were detected. In the last series when blood sampling was taken in two sec intervals, time dependent augmentation of stress glucose elevation was found. The augmentation was more expressed in the rats with free access to diet than in starvated animals.
Highlights
The proper aim of the presented paper is a comparison of different manners of blood sampling when basal glycemia and/or glucose tolerance curve is monitored
When compared basal glycemia obtained under light ether anaesthesia and under Nembutal anaesthesia. statistically significant differences were obtained only in one group of rats, i.e., in SHR lean females (Table l)
The lower level was obtained under decapitation without narcosis.In the mentioned comparison the animal represents the control for itself
Summary
The proper aim of the presented paper is a comparison of different manners of blood sampling when basal glycemia and/or glucose tolerance curve is monitored. Kelsey (2) and Mangili et al (5) when studying the basal ACTH secretion obtained all basal samples under the ether anaesthesia but in less than 90 s before a stress-induced increase in ACTH secretion could occured. Placing the animal for 3 minutes in an ether vapor saturated area (4) is frequently used as a systemic stressor.On the other hand, Kelsay (2) and Mangili et al (5) used ether narcosis when basal ACTH was monitored. It is apparent that ether must be taken into consideration as stressor only under the some condition, i.e., when there is sufficient time delay between the begining of ether vapor inhalation and the blood sampling used for determination of ACTH and/or plasma corticosterone
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