Abstract
A comparison has been made between the published methods of concentrating amino-acids from sea water for analysis. Very poor recoveries were obtained by coprecipitation with ferric hydroxide (Tatsumotoet al. 1961) and by the method of Degenset al. (1964). Recoveries by coprecipitation with the hydroxides of gallium, aluminium and indium were also poor. Recoveries of ∼90% were attained by the procedure of Palmork (1963b); and this has been used in conjunction with thin layer chromatography in a semiquantitative method for the determination of amino-acids. The sea water (2·5 1.) is concentrated to about 15 of its original volume in a climbing-film evaporator. The concentrate is transferred to a rotary-film evaporator and evaporated in vacuo. The salts which crystallize are removed from time to time. The final desalting process is carried out by cation exchange. The amino-acids are separated from one another by thin-layer chromatography and determined photometrically as their ninhydrin compounds. Eleven amino-acids have been determined in water samples from the Irish Sea; their concentrations range from 2 to 16 μg/l.
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