Abstract
In plant genetic engineering, to ensure the efficiently expression of foreign genes in recipient plant cells require high-quality promoters. In this study, a novel promoter, WY172, derived from Oidium heveae B.A. Steinm, was isolated and functionally characterized. GUS staining and GUS activity tests indicated that WY172 could effectively drive GUS expression in both monocotyledonous rice and dicotyledonous tobacco. The micro-hypersensitive response revealed that transgenic tobacco harbouring WY172 and HpaXm had more blue necrotic cells than that of positive control of CaMV35S-HpaXm transgenic tobacco. As well, TMV inoculation tests showed fewer lesions on the leaves of WY172-HpaXm transgenic tobacco than those of the positive control, indicating that WY172 could drive the expression of exogenous functional genes HpaXm. Furthermore, the expression level of the HpaXm gene in WY172-HpaXm transgenic tobacco after TMV inoculation was 7.1 times that of uninoculated tobacco. And WY172 also had a relative stronger ability to drive expression of HpaXm after TMV inoculation compared with non-inoculated plants, indicating that WY172 might be a pathogen-inducible promoter. Bioinformatics analysis indicated that WY172 is rich in pathogen-induced cis-acting elements such as auxin response element and light response elements, and induction experiments demonstrated that WY172 drives GUS expression after exogenous application of IAA, which both supported that WY172 might be an inducible promoter. In conclusion, WY172 promoter is an IAA- and pathogen-inducible promoter, which might has high potential for use in a wide range of hosts. By blasting and predicting the Oidium heveae B.A. Steinm genome, we obtained and preliminarily demonstrated that the WY172 promoter that may be induced by IAA and TMV. WY172 can play a role in rice and tobacco, and can drive efficient expression of HpaXm in transgenic tobacco and produce TMV resistance and micro-hypersensitive response.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
