The detection of tetracycline residues in the liver of Tilapia fish from traditional markets in Yogyakarta using High Performance Liquid Chromatography (HPLC)

Abstract

Antibiotic contamination is detrimental to the aquatic environment of living organisms, such as fish, which are mostly consumed by humans. However, the increase in fish consumption in certain areas should be controlled due to food safety concerns. One of the food safety concerns in Indonesia is the presence of tetracycline residue in fish liver, which can be detected by high-performance liquid chromatography (HPLC). This study aimed to detect tetracycline residue in the liver of tilapia fish from traditional markets in Yogyakarta using HPLC Shimadzu 6.1. The samples were 62 tilapia fish obtained from 16 traditional markets in Yogyakarta and incorporated into the HPLC apparatus at an oven temperature, UV-Vis detector wavelength, flow rate, and amount injection of 30°C, 355 nm, 1 ml/minute, and 20 μl, respectively. The results were presented as chromatograms with peak area, while the positive samples were indicated by the presence of a tetracycline peak area during the 4th minute and 5th minute. The analysis showed that 48.4% of the samples contained tetracycline residue. Also, the presence of tetracycline residues in tilapia livers can be due to antibiotic contamination of the water, the use of antibiotics as a feed additive, or antibiotic contamination of soil ponds.