Abstract
The aim of this research is to detect the PO cattle as superior beef cattle based on conserved sequence of growth hormone gene. DNA of three superior PO bull from BBIB Singosari and ten PO cattles from UPA Pasuruan has been analyzed.. DNA isolation has been conducted by salting out technique, and continued with PCR & sequencing. Based on clustalW allignment using Mega 5.2 software it is seen that among 214bp, out of 329bp read, 58.4% of sequences are same for the 13 cattles; so during almost one hundred years, since Ongole Cattle introduced to Indonesia from Madras (India), there are still 58.4% of the sequences are not changed yet. The conserved sequences are distributed along the growth hormone gene sequence read. The longest conserved sequence is nineteen bp, that is GGGCCTGGGGCGGCCTTCT. The nineteen conserved sequence and others can be used as indicators of superior PO cattle.
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