Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV-2) has infected the human system resulting in Covid-19, and has spread rapidly worldwide. Therefore, a fast, simple, cost-effective, and accurate detecting tool is required. The standard diagnostic tool of the World Health Organization is the reverse transcription-polymerase chain reaction (RT-PCR). This method detects the presence of viral genetic material in the human body with accurate results. However, it has several limitations in terms of equipment, personnel, duration, and cost. Therefore, a fast, simple, and sensitive alternative detection, is required, one of which is the reverse transcription loop-mediated isothermal amplification (RT-LAMP) that functions under isothermal conditions. This method is battery-driven, hence, easy to move closer to the patient. Conclusively, the RT-LAMP test for SARS CoV-2 diagnosis produces comparable sensitivity to a standard RT-PCR and is more suitable for resource-poor settings, such as rural areas of developing countries.
Highlights
At the end of 2019, a new type of virus that infects the human system was discovered and named Severe Acute Respiratory Syndrome Coronavirus-2 (SARSCoV-2) by the World Health Organization, and the associated disease was named coronavirus disease 2019 (Covid-19) [1]
The reverse transcription–polymerase chain reaction is a laboratory technique used to detect the presence of genetic material present in cells and bacteria, including SARS-CoV-2
The material genetic of SARS-CoV-2 is a type of RNA virus, it needs to be converted into DNA using the reverse transcriptase enzyme into cDNA, using the reverse transcription process
Summary
At the end of 2019, a new type of virus that infects the human system was discovered and named Severe Acute Respiratory Syndrome Coronavirus-2 (SARSCoV-2) by the World Health Organization, and the associated disease was named coronavirus disease 2019 (Covid-19) [1]. The inclusion criteria include articles that discussed RT–LAMP or RT–PCR as a diagnostic for SARSCoV-2. The reverse transcription–polymerase chain reaction is a laboratory technique used to detect the presence of genetic material present in cells and bacteria, including SARS-CoV-2. The material genetic of SARS-CoV-2 is a type of RNA virus, it needs to be converted into DNA using the reverse transcriptase enzyme into cDNA, using the reverse transcription process.
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