Abstract
The efficacy of the acridine orange (AO), gram (G), and methylene blue (MB) microscopic procedures was analyzed using positive blood cultures monitored by a radiometric procedure (Bactec) in children. Sixty-eight positive cultures included the following isolates: Haemophilus influenzae type b, Neisseria meningitidis, Streptococcus pneumoniae, Enterobacteriaceae, Staphylococcus aureus, Candida spp., and seven other pathogens. The MB stain yielded the highest detection rate, 99%, in comparison with 94 and 93% for the AO and G stains, respectively. Since the MB stain yielded comparable results to the AO procedure with no requirement for a fluorescent microscope, the former method is recommended for confirming the presence and initial characterization of microorganisms in blood cultures monitored by Bactec from children.
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