The Detection of Inactivated p53 Protein by a Biosensor

Abstract

P53 is a gene that closely correlates with human tumors. In this paper, we explore how various biology techniquescan be applied to detect the malfunction of p53. Firstly, we insert the GFP gene, as correctly translated p53 proteinwill undergo fluorescence. Secondly, we apply artificial enzymes such as an antibody, and the structure of functionalp53 protein differs from those that are incorrectly translated. The enzyme can detect this as its active site matches thestructure of the functional p53 protein. Lastly, we aim to detect not the p53 protein but its side product. Caspase 3 is theone we will target to find. In this paper, we carefully compare and contrast these three methods, and the choice shouldalways be considere with different experimental conditions. The detection using GFP should be the best method amongthe rest, as it gives a visually accessible result, is also easy, and can produce the result very quickly.